Molecular Cytogenetic Core

Overview

The Molecular Cytogenetic Core provides tools for the preparation of human and murine samples suitable for molecular genetic and cytogenetic analysis of the entire genome. These tools include the establishment of EBV transformed cell lines; isolation of DNA and mRNA from a variety of tissue culture samples as well as primary biopsies; preparation of metaphase chromosomes suitable for fluorescence in situ hybridization (FISH) and Spectral Karyotyping (SKY) or whole chromosome paints for human and mouse genome. The core personnel is trained to hybridize commercial probes and to designed locus specific probes for regions of interest to investigators. All the probes are custom designed and in house generated.

Services

  • FISH HYBRIDIZATION: Locus specific probes and chromosome painting (human and mouse) up  to three colors
  • SKY-Spectral Karyotyping (human and mouse)
  • DAPI-Staining and Chromosome Count
  • Generation of Locus Specific Probes (BAC clones, human and mouse)
  • EBV transformation of human lymphocytes
  • DNA Isolation
  • mRNA Isolation
  • Tissue Culture
  • PBMC Isolation and enrichment of other blood cells (T,B and eosinophils)
  • DNA and Cell Lines Repository
  • Picogreen DNA quantification
  • Mycoplasma detection

Location and Contacts

Molecular Cytogenetic Core

Price Center/Block Research Pavillion 413A

 
 
 

Scientific Director
Cristina Montagna, Ph.D.
718.678.1158
cristina.montagna@einstein.yu.edu

Operations Director
Jidong Shan, Ph.D.
718.678.1155
jidong.shan@einstein.yu.edu