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Analytical Imaging Facility

Electron Microscopy

The Analytical Imaging Facility provides complete sample preparation utilizing a broad spectrum of techniques for examination by Transmission Electron Microscopy (TEM) or Scanning Electron Microscopy (SEM). Whether your interests lie in standard morphology or immunohistochemistry our staff will design protocols that will facilitate your experiments and optimize your results. Once the samples are processed, users can either work on the microscopes with one of our staff or independently (following training). Please see or contact one of our staff to plan a future experiment.

Transmission Electron Microscopes

JEOL 100CX II  

100cx microscope
  • 100 kV transmission electron microscope
  • Side entry goniometer stage
  • High image contrast
 

JEOL 1200 EX  

1200cx microscope 

  • 120 kV transmission electron microscope
  • Side entry goniometer stage
  • Minimum dose system
  • Side mounted wide angle Gatan video camera
 

Tecnai 20  

Microscope Tenchi
  • 200 kV transmission electron microscope
  • High brightnessLaB6 filament
  • Low Dose mode for cryoEM
  • Tietz F-224 2k x 2k bottom mount CCD camera
  • Compustage
  • Cryo specimen holders
  • High tilt specimen holders for electron tomography
  • SerialEM
 
 

Scanning Electron Microcope

Zeiss Supra 40 Field Emission SEM

microscope zeiss sem 

  • Operating voltage range from 0.02- 30kV
  • High brightness LaB6  filament
  • Windows based Smart SEM control software
  • Backscatter and STEM detectors
  • OxFord INCA EDS
  • ATLAS- Large area mapping
  • Shuttle and find for correlative fluorescence and SEM
 

Specimen Preparation for Electron Microscopy

The staff of the AIF offers full service sample preparation for many standard and state of the art EM techniques. These include:

  • Embedding utilizing either epoxy or acrylic resins at ambient or low temperatures
  • Thin Sectioning with a Reichert Ultracut E or Leica UCT ultramicrotome
  • Negative Staining of viruses and bacteria 
  • Freeze Fracture using a Cressington CFE-50 Freeze Etch Unit
  • Immunogold Labeling following pre or post embedding protocols
  • Cryoultramicrotomy utilizing a Leica UCT cryoultramicrotome
  • Tokyasu immunogold labeling of ultrathin cryo sections 
  • Critical Point Drying with a Tousimis Samdri 790 Critical Point Dryer
  • Sputter Coating using a Denton Sputter Coater
  • Slam Freeze Cryofixation using a Life Cell CF100 Slam Freezer, which can be followed by High Resolution Rotary Shadowing in a Cressington CFE-50 
  • High Pressure Freezing using a Bal-Tec HPM-010 High Pressure Freezing Machine followed by Freeze Substitution and Low Temperature Embedding in a RMC FS-7500 Freeze Substitution
    Unit 
  • Plunge Freezing of macromolecules for cryoEM using a FEI Vitrobot
 

Preparation for electron microscopy: 

All projects must be approved by Frank Macaluso in advance. Experimental design, special issues (such as dealing with pathogens), and scheduling must be arranged in advance. Preps are done three days per week on a strict schedule. 

 
 

TEM Image Gallery
SEM Image Gallery 

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