Mechanisms of miRNA action: Direct Identification of miRNA Targets using CLIP Methodology
A postdoctoral position is open in the laboratory of Dr. Charles E. rogler to carry out investigations into the mechanisms of miRNA action. This project will focus on understanding miRNA mechanism of action by direct biochemical identification of miRNA targets in liver and liver tumor tissue. The approach will utilize a newly developed protocol called HITS-CLIP published by Chi, S.W. et al, Nature 460:479-486, 2009. The Rogler laboratory has experience in cloning and deep sequencing of miRNAs and their targets and this will be a cutting edge approach to advance our fundamental understanding of miRNA mechanisms of action by direct identification of their targets present in RISC. Applicants should have a broad base of experience in biology, molecular genetics, and biochemistry. Albert Einstein College of Medicine is an equal opportunity employer. Please send CV and names of referees to: firstname.lastname@example.org
Charles E. Rogler, PhD
Professor of Medicine and Microbiology/Immunology
Department of Medicine
Albert Einstein College of Medicine
Jack and Pearl Resnick Campus
1300 Morris Park Avenue
Bronx, NY 10461
Relevant Rogler Laboratory miRNA Publications.
1. Landgraf, P., et al A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing. Cell 129:1401-1414, 2007.
2. Rogler, C.E., Zhou, H.C., LeVoci, L. and Rogler, L.E. Clonal, cultured, murine fetal liver hepatoblasts maintain their liver specification in chimeric mice. Hepatology, 46: 1971-1978, 2007.
3. Connolly, E, Melegari, M., Landgraf, P., Tchaikovskaya, T., Tennant, B.C., Slagle, B.L., Rogler, L.E., Zavolan, M., Tuschl, T. and Rogler, C.E. Elevated expression of the miR-17-92 polycistron and miR-21 in hepatocellular carcinoma contributes to the malignant phenotype. Am J Pathol. 173:856-864, 2008
4. Rogler, C.E. MicroRNAs make inroads into liver development. Gastroenterology 136:770-772, 2009.
5. Rogler, C.E., Levoci, L., Tchaikovskaya, T. and Rogler, L.E. MiR-23b cluster miRNAs regulate TGFβ/BMP signaling and differentiation of liver stem cells by targeting Smads. Hepatology 50: 575-584, 2009.
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