Areas of Research Focus
Develop novel RNA aptamers directed to key viral functions with a view to develop hematopoietic gene therapies for HIV disease.
Delineate structural determinants of the key functions of HIV-1 reverse transcriptase such as dNTP recognition, drug resistance and strand displacement synthesis.
Identifying the viral determinants of HIV associated dementia (HAD) and to understand the mechanistic basis of HAD.
Delineate the mechanism of mammalian telomerase reverse transcriptase functions.
Joshi. P., North, T. W. and Prasad, V. R. (2005) Aptamers Directed to HIV-1 Reverse Transcriptase Display Greater Efficacy over Small Hairpin RNAs Targeted to Viral RNA in Blocking HIV-1 Replication. Mol. Ther.11:677-686.
Fisher, T. S., Joshi, P. and Prasad, V. R. (2005) HIV-1 reverse transcriptase mutations that confer decreased in vitro susceptibility to anti-RT DNA aptamer RT1t49 confer cross resistance to other anti-RT aptamers but not to standard RT inhibitors. AIDS Res. Ther.2:8.
Drosopoulos, W. C., DiRenzo, R. and Prasad, V. R. (2005) Human telomerase RNA template sequence is a determinant of telomere repeat extension rate. J. Biol. Chem. 280: 32801-32810.
Curr, K., Nashiru, O., Lennerstrand, J., Larder, B. A. and Prasad, V. R. (2006) Influence of naturally occurring insertions in the fingers subdomain of human immunodeficiency virus type 1 reverse transcriptase on polymerase fidelity and mutation rates in vitro.J. Gen. Virol.87: 419-428.
Hamburgh, M., Curr, K., Monaghan, M., Rao, V. R., Tripathi, S., Preston, B. D., Sarafianos, S, Arnold, E. A. and Prasad V. R. (2006) Structural determinants of slippage-mediated mutations by human immunodeficiency virus type 1 reverse transcriptase. J. Biol. Chem.281:7421-7428.
Mandal, D., Dash, C. V., Le Grice, S. F. and Prasad V. R. (2006) Analysis of HIV-1 replication block due to substitutions at F61 residue of reverse transcriptase reveals additional defects involving RNase H function. Nuc. Acids Res.34:2853-2863.
Garforth, S., Wu, Y. Y. and Prasad, V. R. (2006) Structural features of mouse telomerase RNA are responsible for the lower activity of mouse telomerase vs. human telomerase. Biochem. J.397:399-406.
Garforth, S., Lee, T. W., Parniak, M. A., Kool, E. T. and Prasad, V. R. (2006) Site-directed mutagenesis in the fingers subdomain of HIV-1 reverse transcriptase reveals a specific role for the b3-b4 hairpin loop in dNTP selection. J. Mol. Biol.365:38-49
Drosopoulos W. C. and Prasad, V. R. (2006) The active site residue Valine 867 in human telomerase reverse transcriptase influences nucleotide incorporation and fidelity. Nuc. Acids Res.2007 Jan 30.