Faculty Profile

Dr. Karen S. Jakes, Ph.D.

Karen S. Jakes, Ph.D.

Research Professor, Department of Physiology & Biophysics

Selected Publications

Jakes KS. Daring to be different: colicin N finds another way. Mol Microbiol. 2014 May;92(3):435-9. doi: 10.1111/mmi.12569. Epub 2014 Mar 19. PubMed PMID: 24589284; PubMed Central PMCID: PMC4051199. 

Jakes KS. Translocation trumps receptor binding in colicin entry into Escherichia coli. Biochem Soc Trans. 2012 Dec 1;40(6):1443-8. doi: 10.1042/BST20120207. Review. PubMed PMID: 23176496. 

Udho E, Jakes KS, Finkelstein A. TonB-dependent transporter FhuA in planar lipid bilayers: partial exit of its plug from the barrel. Biochemistry. 2012 Aug 28;51(34):6753-9. Epub 2012 Aug 15. PubMed PMID: 22846061; PubMed Central PMCID: PMC3448877. 

Jakes KS, Cramer WA. Border crossings: colicins and transporters. Annu Rev Genet. 2012;46:209-31. doi: 10.1146/annurev-genet-110711-155427. Epub 2012 Aug 28. Review. PubMed PMID: 22934645. 

Basilio D, Jennings-Antipov LD, Jakes KS, Finkelstein A. Trapping a translocating protein within the anthrax toxin channel: implications for the secondary structure of permeating proteins. J Gen Physiol. 2011 Apr;137(4):343-56. doi: 10.1085/jgp.201010578. Epub 2011 Mar 14. PubMed PMID: 21402886; PubMed Central PMCID: PMC3068283. 

Jakes KS, Finkelstein A. The colicin Ia receptor, Cir, is also the translocator for colicin Ia. Mol Microbiol. 2010 Feb;75(3):567-78. doi: 10.1111/j.1365-2958.2009.06966.x. Epub 2009 Nov 17. PubMed PMID: 19919671; PubMed Central PMCID: PMC3493618. 

Udho E, Jakes KS, Buchanan SK, James KJ, Jiang X, Klebba PE, Finkelstein A. Reconstitution of bacterial outer membrane TonB-dependent transporters in planar lipid bilayer membranes. Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21990-5. doi: 10.1073/pnas.0910023106. Epub 2009 Dec 3. PubMed PMID: 19959664; PubMed Central PMCID: PMC2799797. 

Kienker PK, Jakes KS, Finkelstein A. Identification of channel-lining amino acid residues in the hydrophobic segment of colicin Ia. J Gen Physiol. 2008 Dec;132(6):693-707. doi: 10.1085/jgp.200810042. PubMed PMID: 19029376; PubMed Central PMCID: PMC2585860. 

Buchanan SK, Lukacik P, Grizot S, Ghirlando R, Ali MM, Barnard TJ, Jakes KS, Kienker PK, Esser L. Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import. EMBO J. 2007 May 16;26(10):2594-604. Epub 2007 Apr 26. PubMed PMID: 17464289; PubMed Central PMCID: PMC1868905. 

Wu Z, Jakes KS, Samelson-Jones BS, Lai B, Zhao G, London E, Finkelstein A. Protein translocation by bacterial toxin channels: a comparison of diphtheria toxin and colicin Ia. Biophys J. 2006 Nov 1;91(9):3249-56. Epub 2006 Aug 11. PubMed PMID: 16905612; PubMed Central PMCID: PMC1614471. 

Kienker PK, Jakes KS, Blaustein RO, Miller C, Finkelstein A. Sizing the protein translocation pathway of colicin Ia channels. J Gen Physiol. 2003 Aug;122(2):161-76. Epub 2003 Jul 14. PubMed PMID: 12860927; PubMed Central PMCID: PMC2229546. 

Zarivach R, Ben-Zeev E, Wu N, Auerbach T, Bashan A, Jakes K, Dickman K, Kosmidis A, Schluenzen F, Yonath A, Eisenstein M, Shoham M. On the interaction of colicin E3 with the ribosome. Biochimie. 2002 May-Jun;84(5-6):447-54. PubMed PMID: 12423788. 

Huster D, Yao X, Jakes K, Hong M. Conformational changes of colicin Ia channel-forming domain upon membrane binding: a solid-state NMR study. Biochim Biophys Acta. 2002 Apr 12;1561(2):159-70. PubMed PMID: 11997116. 

Slatin SL, Nardi A, Jakes KS, Baty D, Duché D. Translocation of a functional protein by a voltage-dependent ion channel. Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1286-91. PubMed PMID: 11830660; PubMed Central PMCID: PMC122182. 

Soelaiman S, Jakes K, Wu N, Li C, Shoham M. Crystal structure of colicin E3: implications for cell entry and ribosome inactivation. Mol Cell. 2001 Nov;8(5):1053-62. PubMed PMID: 11741540. 

Jakes KS. The importance of being cleaved: an essential step in killing by enzymatic colicins. Mol Cell. 2001 Jul;8(1):4-6. PubMed PMID: 11511355. 

Kienker PK, Jakes KS, Finkelstein A. Protein translocation across planar bilayers by the colicin Ia channel-forming domain: where will it end? J Gen Physiol. 2000 Oct;116(4):587-98. PubMed PMID: 11004207; PubMed Central PMCID: PMC2230624. 

Jakes KS, Kienker PK, Finkelstein A. Channel-forming colicins: translocation (and other deviant behaviour) associated with colicin Ia channel gating. Q Rev Biophys. 1999 May;32(2):189-205. Review. PubMed PMID: 10845238. 

Senzel L, Huynh PD, Jakes KS, Collier RJ, Finkelstein A. The diphtheria toxin channel-forming T domain translocates its own NH2-terminal region across planar bilayers. J Gen Physiol. 1998 Sep;112(3):317-24. PubMed PMID: 9725891; PubMed Central PMCID: PMC2229418. 

Jakes KS, Kienker PK, Slatin SL, Finkelstein A. Translocation of inserted foreign epitopes by a channel-forming protein. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4321-6. PubMed PMID: 9539735; PubMed Central PMCID: PMC22487. 

Slatin SL, Qiu XQ, Jakes KS, Finkelstein A. Identification of a translocated protein segment in a voltage-dependent channel. Nature. 1994 Sep 8;371(6493):158-61. PubMed PMID: 7521016. 

Qiu XQ, Jakes KS, Finkelstein A, Slatin SL. Site-specific biotinylation of colicin Ia. A probe for protein conformation in the membrane. J Biol Chem. 1994 Mar 11;269(10):7483-8. PubMed PMID: 8125966. 

Jakes KS, Davis NG, Zinder ND. A hybrid toxin from bacteriophage f1 attachment protein and colicin E3 has altered cell receptor specificity. J Bacteriol. 1988 Sep;170(9):4231-8. PubMed PMID: 3045089; PubMed Central PMCID: PMC211432.

Jakes KS, Zinder ND. Plasmid ColE3 specifies a lysis protein. J Bacteriol. 1984 Feb;157(2):582-90. PubMed PMID: 6319368; PubMed Central PMCID: PMC215286.

Jakes KS, Model P. Mechanism of export of colicin E1 and colicin E3. J Bacteriol. 1979 Jun;138(3):770-8. PubMed PMID: 378936; PubMed Central PMCID: PMC218103.

Ravetch JV, Jakes KS. Intact 3' end of 16S rRNA is not required for specific mRNA binding. Nature. 1976 Jul 8;262(5564):150-3. PubMed PMID: 778638.

Steitz JA, Jakes K. How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8. PubMed PMID: 1107998; PubMed Central PMCID: PMC388805.

Jakes KS, Zinder ND. Highly purified colicin E3 contains immunity protein. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3380-4. PubMed PMID: 4215078; PubMed Central PMCID: PMC433776.

Jakes K, Zinder ND, Boon T. Purification and properties of colicin E3 immunity protein. J Biol Chem. 1974 Jan 25;249(2):438-44. PubMed PMID: 4588566.

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