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Faculty Profile

John S. Condeelis, Ph.D.

Dr. John S. Condeelis

Professor, Department of Anatomy & Structural Biology

Co-Chair, Department of Anatomy & Structural Biology

The Judith and Burton P. Resnick Chair in Translational Research, Department of Anatomy & Structural Biology

Co-Director, Gruss-Lipper Biophotonics Center

Co-Director, Integrated Imaging Program

Director, Tumor Microenvironment and Metastasis Program, AECC

Scientific Director, Analytical Imaging Facility

 

Professional Interests

John Condeelis' research interests are in optical physics, cell biology and biophysics, cancer biology and mouse models of cancer. He and his collaborators developed the multiphoton imaging technology and animal models used to identify invasion and intravasation microenvironments in mammary tumors. This led to the discovery of the paracrine interaction between tumor cells and macrophages in vivo, and the role of macrophages in the migration of tumor cells and their dissemination from primary tumors via blood vessels to distant metastatic sites. Based on these results, cell collection techniques, including the in vivo invasion assay, and markers for FACS, were developed for the collection of migrating and disseminating macrophages and tumor cells. This led to the discovery of the mouse and human invasion signatures. 


John Condeelis has devised optical microscopes for uncaging, biosensor detection and multiphoton imaging for these studies and has used novel caged-enzymes and biosensors to test, in vivo, the predictions of the invasion signatures regarding the mechanisms of tumor cell dissemination and metastasis. This work has supplied markers for the prediction of breast tumor metastasis in humans. Three of these markers, TMEM, MenaCalc and cofilin x p-cofilin, have been used in retrospective studies of cohorts of breast cancer patients to predict metastatic risk and are now in clinical validation trials. He has authored more than 280 scientific papers on various aspects of cell and cancer biology, biophysics and optical imaging.

 

Selected Publications

From over 280 Publications 

Sung Jun Lim, S.J., Zahid, M.U., Le, P., Ma, L., Entenberg, D., Harney, A.S., Condeelis, J., and Smith, A.M. (2015) Brightness-Equalized Quantum Dots. Nature. PMCID: In process

Jimenez, L., Sharma, V.P., Condeelis, J., Harris, T., Ow, T.J., Prystowsky, M.B., Childs, G., and Segall, J.E. (2015) . MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma. Arch Pathol Lab Med. PMID: 26172508/PMCID: In Process

Forse, C.L., Agarwal, S., Pinnaduwage, D., Gertler, F., Condeelis, J., Lin, J., Xue, X., Johung, K., Mulligan, A.M., Rohan, T., Bull, S.B., and Andrulis, I.L. (2015) Menacalc, a quantitative method of metastasis assessment, as a prognostic marker for axillary 4 node-negative breast cancer. BMC Cancer. PMID: 26112005 /PMCID: PMC4482190

Harney, A.S., Arwert, E.N., Entenberg, D., Wang, Y., Guo, P., Qian, B-Z., Oktay, M.,  Pollard, J.W., Jones, J.G., and Condeelis, J.S. (2015) Real-time imaging reveals local, transient vascular permeability and tumor cell intravasation stimulated by Tie2Hi macrophage-derived VEGFA. Cancer Discovery. In Press 

Zolla V, Nizamutdinova IT, Scharf B, Clement CC, Maejima D, Akl T, Nagai T, Luciani P, Leroux JC, Halin C, Stukes S, Tiwari S, Casadevall A, Jacobs WR Jr, Entenberg D, Zawieja DC, Condeelis J, Fooksman DR, Gashev AA, and Santambrogio L. (2015) Aging-related anatomical and biochemical changes in lymphatic collectors impair lymph transport, fluid homeostasis, and pathogen clearance. Aging Cell. 2015 May 15. PMID: 25982749/PMCID: In Process.

Valenzuela-Iglesias A., Bravo-Cordero J.J., Sharma V.P., Beaty B.T., Gutierrez-Millan L.E., Ding Z., Roy P., and Condeelis, J. (2014) Profilin 1 regulates invadopodium maturation in human breast cancer cells.  Eur J Cell Biol. 2015 Feb;94(2):78-89. PMID: 25613364/PMCID: PMC4322761.

Pignatelli, J., Goswami, S., Jones, J., Rohan, T., Pieri, E., Chen, X., Adler, E., Maleki, S., Bresnick, A., Gertler, F., Condeelis, J., and Oktay, M. (2014) Invasive Breast Carcinoma Cells from Patients Exhibit MenaINV-and Macrophage-Dependent Transendothelial Migration. Science Signaling. 7(353):1-12. PMID: 25429076 / PMCID: PMC4266931.

Weitsman, G., Lawler, K., Kelleher, M., Barrett, J., Barber, P., Shamil, E., Festy, F., Patel, G., Fruhwirth, G., Huang, L., Tullis, I., Woodman, N., Ofo, E., Ameer-Beg, S., Irshad, S., Condeelis, J., Gillett, C., Ellis, P., Vojnovic, B., Coolen, A., and Ng, T. (2014) Imaging tumour heterogeneity of the consequences of a PKCα–substrate interaction in breast cancer patients. Biochem. Soc. Trans. 1;42(6):1498-505. PMID: 25399560 / PMCID /PMC4259014

Beaty, B.T. and Condeelis, J. (2014) Digging a little deeper: the stages of invadopodium formation and maturation. Eur. J. of Cell Biol..  PMID: 25113547/PMCID: PMC4262566

Patsialou, A., Wang, Y., Pignatelli, J., Chen, X., Entenberg, D., Oktay, M. and Condeelis, J.C. (2014) Autocrine CSF1R signaling mediates switching between invasion and proliferation downstream of TGFβ in claudin-low breast tumor cells.  Oncogene. PMID: 25088194/PMCID: PMC4317382

Patsialou, A. and Condeelis, J. (2014) Metastatic cells: moving onco-targets. Oncotarget. 5(11):3424-5. PMID: 24907202 / PMCID: PMC 4116491.

Beaty B.T., Wang Y., Bravo-Cordero J.J., Sharma V, Miskolci V., Hodgson L., and Condeelis, J. (2014) Talin regulates moesin-NHE-1 recruitment to invadopodia and promotes mammary tumor metastasis. J. Cell Biol. 205(5):737-51. PMID: 24891603/ PMCID: PMC4050723.

Bergman, A., Condeelis, J. and Gligorijevic, B. (2014) Invadopodia in context.  Cell Adhesion & Migration. 8(3):273-279.  PMID: 24713806 / PMCID: PMC4198352

Bravo-Cordero, J.J., Hodgson, L. and Condeelis, J. (2014) Spatial regulation of tumor cell protrusions by RhoC. Cell Adh. Migr.  8(2) 263-267. PMID: 24642482 / PMCID: PMC4198350

Rohan, T.E., Xue, X., Lin, H-M., Lin, D’Alfonso, T.M., Ginter, P.G, Oktay, M.H., Robinson, B.D., Ginsberg, M., Gertler, F.B., Glass, A.G., Sparano, J.A., Condeelis, J., and Jones, JG. (2014) Tumor Microenvironment of Metastasis and Risk of Distant Metastasis of Breast Cancer. J Natl Cancer Inst. 106(8). PMID: 24895374 / PMCID: PMC 4133559.

Kwon, M., Lee, S.J., Wang, Y., Rybak, Y., Luna, A., Reddy, S., Adem, A., Beaty, B.T., Condeelis, J. and Libutti, S.K. (2014) Filamin A interacting protein 1-like inhibits WNT signaling and MMP expression to suppress cancer cell invasion and metastasis. International Journal of Cancer. 135(1):48-60. DOI: 10.1002/ijc.28662. PMID: 24327474 / PMCID: PMC3991758.

Sharma, V., Beaty, B., Cox, D., Condeelis, J. and Eddy, R. (2014) An in vitro one-dimensional assay to study growth factor-regulated tumor cell-macrophage interaction.  Methods Mol. Biol.  1172:115-23. Chapter 10 Vol. 1172. PMID: 24908299 / PMCID: PMC4124813/NIHMSID # 605152.

Gligorijevic, B., Bergman, A. and Condeelis, J. (2014) Multiparametric classification links tumor microenvironments with tumor cell phenotype. PLoS Biol.  12(11) e1001995. PMID: 25386698/ PMCID: PMC4227649

Moshfegh, Y., Bravo-Cordero, J.J., Miskolci, V., Condeelis, J., and Hodgson, L. (2014) A Trio-Rac1-PAK1 signalling axis drives invadopodia disassembly. Nature Cell Biol. 16(6):574-86. PMID: 24859002 / PMCID: PMC4083618. Reference number: NCB-H24591A; R1.

Roh-Johnson, M., Bravo-Cordero, J.J., Patsialou, A.P., Sharma, V.P., Guo P., Liu, H., Hodgson, L., and Condeelis, J. (2014) Macrophage contact induces RhoA GTPase signaling to trigger tumor cell intravasation. Oncogene. 33(33):4203-12. doi:10.1038/onc.2013.377. PMID: 24056963 / PMCID: PMC3962803.

Zhou, Z., Sharma, V., Beaty, B., Roh-Johnson, M., Peterson E.A., van Rooijen, N., Kenny, P., Wiley, HS., Condeelis, J. and Segall, J. (2014) Autocrine HBEGF expression promotes breast cancer intravasation, metastasis and macrophage-independent invasion in vivo. Oncogene. DOI: 10.1038/onc.2013.363. PMID: 24013225 / PMCID: PMC3950352

Ding, Z., Joy, M., Bhargava, R., Gunsaulus, M., Lakshman, N., Miron-Mendoza, M., Petroll, M., Condeelis, J., Wells, A., and Roy, P. (2014) Profilin-1 downregulation has contrasting effects on early vs late steps of breast cancer metastasis. Oncogene. 33(16):2065-74. doi: 10.1038/onc.2013.166. PMID: 23686314 / PMCID: PMC3834125.

Tania, N., Condeelis, J., and Edelstein-Keshet, L. (2013) Modeling the synergy of cofilin and Arp2/3 in lamellipodial protrusive activity. Biophys. J. 105(9):1946-55. PMID: 24209839 / PMCID: PMC3824550.

Stadler, S.C., Vincent, C.T., Fedorov, V.D., Patsialou, A., Cherrington, B.D., Wakshlag, J.J., Mohanan, S., Zee, B.M., Zhang, X., Garcia, B.A., Condeelis, J., Brown, A.M, Coonrod, S.A. and Allis, C.D. (2013)  Dysregulation of PAD4-mediated citrullination of nuclear GSK3β activates TGF-β signaling and induces epithelial-to-mesenchymal transition in breast cancer cells. PNAS.  110(29):11851-6. PMID: 23818587 / PMCID: PMC3718105.

Das, S., Sarrou, E., Podgrabinska, S., Cassella, M., Mungamuri, S.K., Feirt, N., Gordon, R., Nagi, C.S., Wang, Y., Entenberg, D., Condeelis, J., and Skobe, M. (2013) Tumor cell entry into the lymph node is controlled by CCL1 chemokine expressed by lymph node lymphatic sinuses. J. Exp. Med. 210(8):1509-28. PMID: 23878309/PMCID: PMC3727324

Bravo-Cordero, J.J., Magalhaes, M., Eddy, R., Hodgson, L., and Condeelis, J. (2013) Functions of cofilin in cell locomotion and invasion. Nature Rev. Mol. Cell Biol. 14(7):405-15. PMID: 23778968/PMCID:  PMC3878614.

Bravo-Cordero, J.J., Sharma, V., Roh-Johnson, M., Chen, X., Eddy, R., Condeelis, J. and Hodgson, L. (2013) Spatial regulation of RhoC activity defines protrusion formation in migrating cells. J. Cell Sci.  126(Pt 15):3356-69. PMID: 23704350/PMCID: PMC0370245

Sharma, V., Eddy, Entenberg, D., R., Kai, M., Gertler, F., and Condeelis, J. (2013) Tks5 and SHIP2 regulate invadopodium maturation, but not initiation, in breast carcinoma cells. Current Biol. 23(21):2079-89.  PMID: 24206842/PMCID: PMC38882144

Patsialou, A., Bravo-Cordero, J.J., Wang, Y., Entenberg, D., Liu, H., Clarke, M.F., and Condeelis, J. (2013) Intravital multiphoton imaging reveals multicellular streaming as a crucial component of in vivo cell migration in human breast tumors. IntraVital. 2(2):e25294. PMID:25013744/PMCID: PMC3908591

Ishihara D, Dovas A, Hernandez, L., Pozzuto M, Wyckoff, J., Segall, J., Condeelis, J., Bresnick AR and Cox, D. (2013) WASp Regulates Leukocyte-Dependent Breast Cancer Metastasis. Cell Rep. 4(3):429-436.  PMID: 23911287/PMCID: PMC3777703.

Sharma, V.P., Entenberg, D., and Condeelis, J. (2013) High-resolution live-cell imaging and time-lapse microscopy of invadopodium dynamics and tracking analysis. Methods Mol. Biol.  1046:343-57. PMID: 23868599/PMCID: PMC3933219.

Bravo-Cordero, J.J., Moshfegh, Y., Condeelis, J. and Hodgson, L. (2013) Live cell imaging of RhoGTPase biosensors in tumor cells. Methods in Mol. Biol. Chapter 22, Vol. 1046:359-70.  PMID: 23868600/PMCID: PMC3784404.

Beaty, B., Sharma, V., Bravo-Cordero, J.J., Simpson, M.A., Eddy. R.J., Koleske, A.J. and Condeelis, J. (2013) β1 integrin regulates Arg to promote invadopodial maturation and matrix degradation. Mol. Biol. Cell. 24(11):1661-75. PMID: 23552693/PMCID: PMC3667720.

Entenberg, D., Kedrin, D., Wyckoff, J., Sahai, E., Condeelis, J., and Segall, J. (2013) Imaging Tumor Cell Movement In Vivo.  Current Protocols in Cell Biol. Chapter 19: Unit 19.7.  doi: 10.1002/0471143030.cb1907s58. PMID: 23456602 / PMCID: 3722061.

Gil-Henn H, Patsialou A, Wang Y, Warren MS, Condeelis, J., and Koleske AJ. (2013) Arg/Abl2 promotes invasion and attenuates proliferation of breast cancer in vivo. Oncogene. 32(21):2622-30.  PMID:22777352 / PMCID: PMC3473103.

Dovas, A., Patsialou, A., Harney, A.S., Condeelis, J., and Cox, D. (2013) Imaging interactions between macrophages and tumour cells that are involved in metastasis in vivo and in vitro. J. Microscopy. 251(3):261-9. doi: 10.1111/j.1365-2818.2012.03667.x. PMID: 23198984 / PMCID: PMC4000025.

Zervantonakis, I.K., Hughes-Alford, S.K., Charest, J.L., Condeelis, J., Gertler, F.B., and Kamm, R.D. (2012) Three-dimensional microfluidic model for tumor cell intravasation and endothelial barrier function. PNAS. 109(34):13515-20. PMID: 22869695 / PMCID: PMC3427099.

Subach, O., Entenberg, D., Condeelis, J. and Verkhusha, V. (2012) A FRET-Facilitated Photoswitching Using an Orange Fluorescent Protein with the Fast Photoconversion Kinetics. J. of the American Chem. Soc. 134(36):14789-99. PMID: 22900938 / PMCID: PMC3444247.

Smirnova, T., Zhou, Z.N., Flinn, R.J., Wyckoff, J., Boimel, P.J., Pozzuto, M., Coniglio, S.J., Backer, J.M., Bresnick, A.R., Condeelis, J.S., Hynes, N.E., and Segall, J.E. (2012). Phosphoinositide 3-kinase signaling is critical for ErbB3-driven breast cancer cell motility and metastasis. Oncogene 31(6):706-715.  PMID: 21725367 / PMCID: PMC3469325.

Raja, W.K., Padgen, M.R., Williams, J.K., Gertler, F.B., Wyckoff, J.B., Condeelis, J.S., and Castracane, J. (2012). Development Path and Current Status of the NANIVID:  A New Device for Cancer Cell Studies. Journal of Micro/Nanolithography, MEMS, and MOEMS (JM3) 11(1), 0130131-9.  NIHMSID: 599555.

Oktay, M.H., Gertler, F.B., Liu, Y.F., Rohan, T.E., Condeelis, J.S., and Jones, J.G. (2012). Correlated immunohistochemical and cytological assays for the prediction of hematogenous dissemination of breast cancer. J Histochem Cytochem 60(3), 168-173.  PMID: 22215635 / PMCID: PMC3351127.

Gligorijevic, B., Wyckoff, J., Yamaguchi, H., Wang, Y., Roussos, E.T., and Condeelis, J. (2012). N-WASP-mediated invadopodium formation is involved in intravasation and lung metastasis of mammary tumors. J Cell Sci 125, 724-734.  PMID: 22389406 / PMCID: PMC3367832.

Bravo-Cordero, J.J., Hodgson, L., and Condeelis, J. (2012). Directed cell invasion and migration during metastasis. Curr Opin Cell Biol 24, 277-283. PMCID:PMC3320684

Bragado, P., Sosa, M.S., Keely, P., Condeelis, J., and Aguirre-Ghiso, J.A. (2012). Microenvironments dictating tumor cell dormancy. Recent Results Cancer Res 195, 25-39.  PMID: 22527492 / PMCID: PMC3516301.

Boimel, P.J., Smirnova, T., Zhou, Z.N., Wyckoff, J., Park, H., Coniglio, S.J., Qian, B.Z., Stanley, E.R., Cox, D., Pollard, J.W., Muller, W.J., Condeelis, J., and Segall, J.E. (2012). Contribution of CXCL12 secretion to invasion of breast cancer cells. Breast Cancer Res 14(1), R23. PMID: 22314082 / PMCID: PMC3496141. 

Bravo-Cordero, J.J., Oser, M., Chen, X., Eddy, R., Hodgson, L., and Condeelis, J. (2011). A novel spatiotemporal RhoC activation pathway locally regulates cofilin activity at invadopodia. Curr Biol 21, 635-644. PMCID: PMC3081966.

Dovas, A., Gligorijevic, B., Chen, X., Entenberg, D., Condeelis, J., and Cox, D. (2011). Visualization of actin polymerization in invasive structures of macrophages and carcinoma cells using photoconvertible beta-actin-Dendra2 fusion proteins. PLoS One 6, e16485. PMCID: PMC3038862.

Entenberg D, Wyckoff J, Gligorijevic B, Roussos ET, Verkhusha VV, Pollard JW, Condeelis, J., (2011) Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging. Nat Protoc. 6(10):1500-20.  PMID: 21959234 / PMCID: PMC4058841.

Gertler F, Condeelis, J., (2011) Metastasis: tumor cells becoming MENAcing. Trends Cell Biol. 21(2):81-90.  PMID: 21071226 / PMCID: PMC3402095.

Hernandez L, Magalhaes M.A., Coniglio S.J., Condeelis, J., Segall J.E. (2011) Opposing roles of CXCR4 and CXCR7 in breast cancer metastasis. Breast Cancer Res. 13(6):R128.  PMID: 22152016 / PMCID: PMC3326570.

Mader CC, Oser M, Magalhaes MA, Bravo-Cordero JJ, Condeelis, J., Koleske AJ, Gil-Henn H. (2011) An EGFR-Src-Arg-cortactin pathway mediates functional maturation of invadopodia and breast cancer cell invasion. Cancer Res. 71(5):1730-41.  PMID: 21257711 / PMCID: PMC3057139.

Magalhaes MA, Larson DR, Mader CC, Bravo-Cordero JJ, Gil-Henn H, Oser M, Chen X, Koleske AJ, Condeelis, J., (2011) Cortactin phosphorylation regulates cell invasion through a pH-dependent pathway. J Cell Biol. 195(5):903-20.  PMID: 22105349 / PMCID: PMC3257566.

 

More Information About Dr. John Condeelis

Condeelis Laboratory

Gruss Lipper Biophotonics Center

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Contact

Albert Einstein College of Medicine
Michael F. Price Center
1301 Morris Park Avenue , Room 220
Bronx, NY 10461

Tel: 718.678.1126
john.condeelis@einstein.yu.edu

 
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Media Coverage

WABC-TV interviews Dr. John Condeelis about his imaging research that is helping to explain how cancer spreads from the primary tumor.

Nature highlights research by Dr. John Condeelis that uses intravital imaging to reveal how breast cancer cells spread from the primary tumor.

More media coverage