Faculty Profile

Dr. John S. Condeelis, Ph.D.

John S. Condeelis, Ph.D.

Professor, Department of Anatomy & Structural Biology

Professor, Department of Surgery

Co-Chair, Department of Anatomy & Structural Biology

The Judith and Burton P. Resnick Chair in Translational Research, Department of Anatomy & Structural Biology

Co-Director, Gruss-Lipper Biophotonics Center

Co-Director, Integrated Imaging Program

Director, Tumor Microenvironment and Metastasis Program, AECC

Scientific Director, Analytical Imaging Facility

Areas of Research: Interests are in optical physics, cell biology and biophysics, cancer biology and mouse models of cancer. Development of the multiphoton imaging technology used to identify intravasation microenvironments in metastatic tumors

Professional Interests

John Condeelis' research interests are in optical physics, cell biology and biophysics, cancer biology and mouse models of cancer. He and his collaborators developed the multiphoton imaging technology and animal models used to identify invasion and intravasation micro-environments in mammary tumors. Integration of intravital multiphoton imaging with computational /systems analysis of living breast tumors identified the dominant tumor cell phenotypes contributing to invasion and dissemination during metastasis. This led to the discovery and verification of the paracrine interaction between tumor cells and macrophages in vivo, the role of macrophages in the migration of tumor cells during HGF-dependent tumor cell streaming to blood vessels and the mechanism of tumor cell dissemination from primary tumors via TMEM (Tumor MicroEnvironment of Metastasis) to distant metastatic sites. Based on these results, cell collection techniques, including the in vivo invasion assay were developed for the collection of migrating and disseminating macrophages and tumor cells. This led to the discovery of the mouse and human invasion signatures, and the TMEM, MenaCalc and MenaINV markers for assessing risk of metastasis and prediction of response in breast cancer patients to both chemotherapy, and receptor tyrosine kinase and tyrosine kinase inhibitors used to suppress metastasis.

John Condeelis has devised optical microscopes for uncaging, biosensor detection and multiphoton imaging for these studies and has used novel caged-enzymes and biosensors to test, in vivo, the predictions of the invasion signatures regarding the mechanisms of tumor cell dissemination and metastasis. He is one of the founding co-directors of the Integrated Imaging Program dedicated to the integration and validation of clinical imaging platforms, including digital pathology, with high resolution optical imaging in the Gruss Lipper Biophotonics Center. He has authored more than 300 scientific papers on various aspects of his research.

Selected Publications

Selected Publications from the last 3 years

Karagiannis GS, Condeelis JS, Oktay MH. (2018) Chemotherapy-induced metastasis: mechanisms and translational opportunities. Clinical & Experimental Metastasis. Jan 6. [PMCID: PMC5762276]

Genna, A., Lapetina, S., Lukic, N., Twafra, S., Meirson, T., Sharma, V.P., Condeelis, J.S. and Gil-Henn, H (2018) Pyk2 and FAK differentially regulate invadopodia formation and function in breast cancer cells. Journal of Cell Biology. 217(1):375-395. [PMCID: PMC5748976]

Karagiannis GS, Condeelis JS, Oktay MH. (2017)Chemotherapy-induced metastasis in breast cancer. Oncotarget, 8(67):110733-110734 [PMCID: PMC5762276]  

Linde N, Casanova-Acebes M, Sosa MS, Mortha A, Rahman A, Farias E, Harper K, Tardio E, Reyes Torres I, Jones J, Condeelis J, Merad M, Aguirre-Ghiso JA. (2018) Macrophages orchestrate breast cancer early dissemination and metastasis. Nat Commun. 9(1):21. [PMCID: PMC5750231] 

Roh-Johnson M, Shah AN, Stonick JA, Poudel KR, Kargl J, Yang GH, di Martino J, Hernandez RE, Gast CE, Zarour LR, Antoku S, Houghton AM, Bravo-Cordero JJ, Wong MH, Condeelis J, Moens CB. (2017) Macrophage-Dependent Cytoplasmic Transfer during Melanoma Invasion In Vivo. Developmental Cell. 4;43(5):549-562.e6. [PMCID: PMC5728704] 

Entenberg D, Voiculescu S, Guo P, Borriello L, Wang Y, Karagiannis GS, Jones J, Baccay F, Oktay M, Condeelis J. (2017) A permanent window for the murine lung enables high-resolution imaging of cancer metastasis. Nature Methods. 15(1):73-80 [PMCID: PMC5755704]

Sparano JA, Gray R, Oktay MH, Entenberg D, Rohan T, Xue X, Donovan M, Peterson M, Shuber A, Hamilton DA, D'Alfonso T, Goldstein LJ, Gertler F, Davidson NE, Condeelis J, Jones J. (2017) A metastasis biomarker (MetaSite Breast™ Score) is associated with distant recurrence in hormone receptor-positive, HER2-negative early-stage breast cancer. NPJ Breast Cancer. 8;3:42.[PMCID: PMC5678158]

Donnelly, S.K., Cabrera, R., Mao, S.P.H., Christin, J.R., Wu, B., Guo, W., Bravo-Cordero, J.J., Condeelis, J.S., Segall, J.E., and Hodgson, L. (2017) Rac3 regulates breast cancer invasion and metastasis by controlling adhesion and matrix degradation. Journal of Cell Biology 4;216(12):4331-4349. [PMCID: PMC5716284]

Entenberg D, Pastoriza JM, Oktay MH, Voiculescu S, Wang Y, Sosa MS, Aguirre-Ghiso J, Condeelis J.(2017) Time-lapsed, large-volume, high-resolution intravital imaging for tissue-wide analysis of single cell dynamics. Methods. 1;128:65-77. [PMCID:PMC5659295]

Harney, A.S., Karagiannis, G.S., Pignatelli, J., Smith, W.C., Kadioglu, E., Wise, S.C., Hood, M.M., Kaufman, M.D., Leary, C.B., Lu, W-P, Al-Ani, G., Chen, M., Entenberg, D., Oktay, M.O., Wang, Y., Chun, L., De Palma, M., Jones, J.G., Flynn, D.L. and Condeelis, J.S. (2017) The selective Tie inhibitor rebastinib blocks recruitment and function of Tie2Hi macrophages in breast cancer and pancreatic neuroendocrine tumors. Molecular Cancer Therapeutics. 16(11):2486-2501. [PMCID: PMC5669998]

Jiang C, Ding Z., Joy M., Chakraborty S., Kim S., Bottcher R., Condeelis J., Singh S., Roy P. (2017) A balanced level of profilin-1 promotes stemness and tumor-initiating potential of breast cancer cells Cell Cycle [PMCID: PMC5788419]

Karagiannis GS, Pastoriza JM, Wang Y, Harney AS, Entenberg D, Pignatelli J, Sharma VP, Xue EA, Cheng E, D'Alfonso TM, Jones JG, Anampa J, Rohan TE, Sparano JA, Condeelis JS, Oktay MH. (2017) Neoadjuvant chemotherapy induces breast cancer metastasis through a TMEM-mediated mechanism. Science Translational Medicine 5;9(397). [PMCID: PMC5592784]

Brian J. Rosenberg, B.J., Gil-Henn, H., Mader, C.C., Halo, T., Yin, T., Condeelis, J.C., Machida, K., Yu, Y.I. and Koleske, A.J. (2017) Phosphorylated cortactin recruits Vav2 guanine nucleotide exchange factor to activate Rac3 and promote invadopodial function in invasive breast cancer cells. Molecular Biology of the Cell. 15;28(10). [PMCID: PMC5426849]

Eddy, RJ, Weidmann, M and Condeelis, J.S. (2017) Tumor cell invadopodia: Invasive protrusions that orchestrate metastasis. Trends in Cell Biology. [PMCID: PMC5524604]

Fluegen G, Avivar-Valderas A, Wang Y, Padgen MR, Williams JK, Nobre AR, Calvo V, Cheung JF, Bravo-Cordero JJ, Entenberg D, Castracane J, Verkhusha V, Keely PJ, Condeelis J and Aguirre-Ghiso JA.(2016) Phenotypic heterogeneity of disseminated tumour cells is preset by primary tumour hypoxic microenvironments. Nature Cell Biology 19(2):120-132. [PMCID: PMC5342902]

Harper KL, Sosa MS, Entenberg D, Hosseini H, Cheung JF, Nobre AR, Avivar- Valderas A, Nagi.C, Girnius N, Davis RJ, Farias EF, Condeelis J, Klein CA, and Aguirre-Ghiso JA. (2016) Mechanism of early dissemination and metastasis in HER2+ mammary cancer. Nature 540, 588–592. [PMCID: PMC5471138]

Pignatelli J, Bravo-Cordero JJ, Roh-Johnson M, Gandhi SJ, Wang Y, Chen X, Eddy RJ, Xue A, Singer RH, Hodgson L, Oktay MH and Condeelis JS (2016) Macrophage-dependent tumor cell intravasation is mediated by Notch 1/MenaINV-initiated invadopodium formation. Scientific reports. 6:37874. [PMCID: PMC5129016]

Leung E, Xue A, Wang Y, Rougerie P, Sharma V, Eddy, R., Cox, D. and Condeelis, J.S. (2016) Blood vessel endothelium - directed tumor cell streaming in breast tumors requires the HGF/C-Met signaling pathway. Oncogene. [NIHMSID: NIHMS820864]

Weidmann MD, Surve CR, Eddy RJ, Gertler FB, Sharma V., and Condeelis, J.S. (2016) Mena INV dysregulates cortactin phosphorylation to promote invadopodium maturation. Scientific Reports. 8;6:36142. [PMCID:PMC5099927]

Wang, Y., Wang, H., Entenberg, D., Xue, A., Wang, W. and Condeelis, J.S. (2016) Direct visualization of the phenotype of hypoxic tumor cells at single cell resolution in vivo using a new hypoxia probe. Intravital. 5(2) e1187803 [PMCID:PMC5079291]

Williams, J.K, Entenberg, D.E., Wang, Y, Avivar-Valderase, A, Padgena, M, Clark, A, Aguirre-Ghiso, J.A, Castracane, J, and Condeelis, J.S. (2016) Validation of a device for the active manipulation of the tumor microenvironment during intravital imaging. Intravital. 5(2) e1182271 [PMCID:PMC4238296]

Rodriguez-Tirado, C., Kitamura, T., Kato, Y., Pollard, J.W., Condeelis, J.S., and Entenberg, D.E. (2016) Long-term high-resolution intravital microscopy in the lung with a vacuum stabilized imaging window. Journal of Visualized Experiments. 6(116) PMID:27768066/

Szulczewski, J.M., Inman, D.R., Entenberg, D.E., Ponik, S.M., Aguirre-Ghiso, J., Castracane, J., Condeelis, J.S., Eliceiri, K.E., & Keely, P.J. (2016) In vivo visualization of stromal macrophages via label-free FLIM-based metabolite imaging. Scientific Reports. 25(6) [PMCID:PMC4879594]

Balsamo M, Mondal C, Carmona, G, McClain LM, Riquelme DN, Tadros J, Ma D, Vasile E, Condeelis JS, Lauffenburger DA, Gertler FB. (2016) The alternatively-included 11a sequence modifies the effects of Mena on actin cytoskeletal organization and cell behavior. Scientific Reports. 17;6:35298. [PMC5066228] 

Morris, B.A., Burkel, B, Ponik, S.M., Fan, J, Condeelis, J., Aguire-Ghiso, J, Castracane, J, Denu, J.M., and Keely, P.J. (2016) Collagen Matrix Density Drives the Metabolic Shift in Breast Cancer Cells. EBioMedicine. (16)30467-4. [PMC5264313]

Harney, A.S., Entenberg, D.E., Wang, Y and Condeelis, J.S. (2016) Extended time-lapse intravital imaging of real-time multicellular dynamics in the tumor microenvironment. Journal of Visualized Experiments. 12(112). [PMCID: PMC4927790]

Karagiannis, G.S, Goswami, S., Jones, J.G., Oktay, M.H., and Condeelis, J.S (2016) Signatures of breast cancer metastasis at a glance. Journal of Cell Science. 129, 1751-1758. [PMCID: PMC48993654]

Khalil, B.D., Hsueh, C., Cao, Y., Abi Saab, A.F., Wang, Y., Condeelis, J.S., Bresnick, A.R., and Backer, J.B. (2016) GPCR signaling mediates tumor metastasis via PI3Kβ. Cancer Research. [PMCID: PMC4915222]

Carmona, G., Perera, U., Gillett, C., Naba1, A., Law, A-L., Sharma, V.P., Wang, J., Wyckoff, J., Balsamo, M., Mosis, F., De Piano, M., Monypenny, J., Woodman, N., McConnell, R.E., Mouneimne, G., Van Hemelrijck, M., Cao, Y., Condeelis, J., O. Hynes, R., Gertler, F.B., and Krause, M. (2016) Lamellipodin promotes invasive 3D cancer cell migration via regulated interactions with Ena/VASP and SCAR/WAVE. Oncogene. 35(39):5155-69.[PMC5031503]

 

More Information About Dr. John Condeelis

Condeelis Laboratory

Gruss Lipper Biophotonics Center

Material in this section is provided by individual faculty members who are solely responsible for its accuracy and content.

Albert Einstein College of Medicine
Michael F. Price Center
1301 Morris Park Avenue , Room 220
Bronx, NY 10461

Tel: 718.678.1126

Research Information

In the News

New England Journal of Medicine highlights research by Drs. Maja Oktay, John Condeelis and George Karagiannis on the mechanism by which some breast cancer tumor cells resist chemotherapy and go on to metastasize.

STAT interviews Dr. John Condeelis about his role in a recent study that linked preoperative chemotherapy with activating the microenvironment of metastasis in breast cancer.

More media coverage