Research Assistant Professor, Department of Microbiology & Immunology
We study the role of metabolic plasticity of pathogens in their interaction with the host. Our model system is Mycobacterium, a genus that is very diverse and highly successful in persisting in humans, animals and the environment. Using an integrated approach of genetic engineering, metabolomics, biochemical assays, transcriptomics and animal experiments we are pursuing fundamental questions such as which nutrients and energy sources do pathogenic microorganisms use in the host, or how do aerobic pathogens survive in host tissues that are hypoxic. Our ultimate goal is to translate our findings into new chemotherapeutic strategies against human pathogens.
02/08/16: Our study "Central Role of Pyruvate Kinase in Carbon Co-Catabolism of Mycobacterium tuberculosis." conducted in collaboration with Dr. John S. Blanchard has just been accepted for publication in the Journal of Biological Chemistry.
02/03/16: Grant success! We have just been awarded funding from the NIH for the project:"Inhibition of methionine biosynthesis eliminates persistent M. tuberculosis"
07/28/15: Our paper "Essential Roles of Methionine and S-adenosylmethionine in the Autarkic Lifestyle of Mycobacterium tuberculosis" has just been published in PNAS. Read the research roundup here.
Central Role of Pyruvate Kinase in Carbon Co-Catabolism of Mycobacterium tuberculosis. Noy T, Vergnolle O, Hartman TE, Rhee KY, Jacobs WR, Berney M*, Blanchard JS*. Journal of Biological Chemistry 2016; http://www.jbc.org/content/early/2016/02/08/jbc.M115.707430.abstract
Cook GM, Hards K, Dunn E, Heikal A, Nakatani Y, Greening C, Crick DC, Fontes FL, Pethe K, Hasenoehrl E and Berney M. OXPHOS as a target space for tuberculosis: success, caution, and future directions. Tuberculosis and the Tubercle Bacilli, Second Edition, ASM PRESS, (IN PRESS 2016)
Berney M*, Berney-Meyer L, Wong KW, Wang J, Chen B, Chen M, Kim J, Harris D, Parkhill J, Chan J, Wang F, Jacobs WR*. Essential Roles of Methionine and S-adenosylmethionine in the Autarkic Lifestyle of Mycobacterium tuberculosis. PNAS 2015; http://www.pnas.org/content/early/2015/07/27/1513033112
Hards K, Robson J, Berney M, Shaw L, Andries K, Koul A, Bald D, Cook GM. Bactericidal mode of action of Bedaquiline. Journal of Antimicrobial Chemotherapy IN PRESS (2015)
Aung HL, Dixon LL, Smith LJ, Sweeney NP, Robson JR, Berney M, Buxton RS, Green J, Cook GM. Novel regulatory roles of cAMP receptorproteins (CRPs) in fast-growing environmental mycobacteria. Microbiology-SGM 2015;161:648-661
Greening C, Constant P, Hards K, Morales S, Oakeshott J, Russell R, Taylor M, Berney M, Conrad R, Cook GM. Atmospheric hydrogen scavenging: from enzymes to ecosystems. Applied and Environmental Microbiology 2015 Feb;81(4):1190-9.
Cook GM, Greening C, Hards K, Berney M. Energetics of pathogenic bacteria and opportunities for drug development. Advances in Microbial Physiology 2014;65:1-62. doi:10.1016/bs.ampbs.2014.08.001.
Hartman T, Weinrick B, Vilcheze C, Berney M, Tufariello J, Cook GM, Jacobs WR. Succinate dehydrogenase is the regulator of respiration in Mycobacterium tuberculosis. PLoS Pathogens 2014 Nov 20; 10.1371/journal.ppat.1004510
Berney M*, Greening C, Conrad R, Jacobs WR, Cook GM. An obligately aerobic soil bacterium activates fermentative hydrogen production to survive reductive stress during hypoxia. PNAS 2014 : 1407034111v1-201407034. Featured with an interview in The Pathologist (https://thepathologist.com/issues/0114/the-survival-artists/)
Pecsi I, Hards K, Ekanayaka NV, Berney M, Hartman TE, Jacobs WR., Cook GM. Essentiality of Succinate Dehydrogenase in Mycobacterium smegmatis and its Role in the Generation of the Membrane Potential under Hypoxia mBio 2014 5(4):e01093-14
Greening C, Villas-Bôas SG, Robson J, Berney M, Cook GM. The growth and survival of Mycobacterium smegmatis is enhanced by co-metabolism of atmospheric H2. PLoS ONE 2014 Jul 24;9(7):e103034.
Berney M*, Hartman T, Jacobs WR*. A Mycobacterium tuberculosis cytochrome bd oxidase mutant is hypersensitive to bedaquiline. mBio 2014; Vol. 5, No. 4, mBio01275-14
Aung HL, Berney M, Cook GM. Hypoxia-activated cytochrome bd expression in Mycobacterium smegmatis is cAMP receptor protein (CRP)-dependent. Journal of Bacteriology Epub 2014 June 16
Greening C, Berney M, Hards K, Cook GM, Conrad R. A soil actinobacterium scavenges atmospheric H2 using two membrane-associated, oxygen-dependent [NiFe] hydrogenases. PNAS 2014 111 (11) 4257-4261; Epub 2014 March 3, doi:10.1073/pnas/1320586111
Berney M*, Cook GM*. Respiration and Oxidative Phosphorylation in Mycobacteria. In: The Structural Basis of Biological Energy Generation, Springer, Dordrecht 2014; 39:277-293
Lagautriere T, Bashiri G, Paterson NG, Berney M, Cook GM, Baker EN. Characterization of the proline utilization pathway in Mycobacterium tuberculosis through structural and functional studies. Acta Crystallographica Section D 2014 70(4): Pages 968-980
Cook GM, Hards K, Vilcheze C, Hartman T, Berney M. Energetics of Respiration and Oxidative Phosphorylation in Mycobacteria. In Molecular Genetics of Mycobacteria, Hatfull, G. and Jacobs, Jr., W.R. (Eds) ASM Press, Washington DC. (2014). Available online in Microbiology Spectrum 2014; 2(3):MGM-0015-2013
Berney M, Greening C, Hards K, and Cook GM. Three [NiFe] Hydrogenases confer metabolic flexibility in the obligate aerobe Mycobacterium smegmatis. Environmental Microbiology 2014 16(1): 318-330. Epub 2013 Nov 25
Berney M*, Weimar MR, Heikal A, Cook GM*. Regulation of Proline Metabolism in Mycobacteria and Its Role in Carbon Metabolism under Hypoxia. Molecular Microbiology 2012 84(4): 664-681; Epub 2012 April 22
McKenzie JL, Robson JR., Berney M, Smith T, Ruthe A, Arcus VL and Cook GM. A VapBC Toxin-Antitoxin Module is a Post-Transcriptional Regulator of Metabolic Flux in Mycobacteria. Journal of Bacteriology 2012 194(9): 2189-2204; Epub 2012 Feb 24
Taniguchi N, Suzuki T, Berney M,Yoshida M, Cook GM. The Regulatory C-terminal Domain of Subunit e of FoF1 ATP Synthase is Dispensable for Growth and Survival of Escherichia coli. Journal of Bacteriology 2011 April;193(8): 2046-2052.
Hammes F, Berney M, Egli T. Cultivation-independent assessment of bacterial viabilityAdvances in Biochemical Engineering and Biotechnology, High Resolution Microbial Single Cell Analytics 2011 124:123-150.
Berney M* and GM Cook*. Unique Flexibility in Energy Metabolism Allows Mycobacteria to Combat Starvation and Hypoxia. PLoS One 2010 Jan;5: e8614
Hümpel A., Gebhard, S., Cook GM, Berney M*. The SigF Regulon in Mycobacterium smegmatis Reveals Roles in Adaptation to Stationary Phase, Heat and Oxidative Stress. Journal of Bacteriology 2010 May;192(10):2491-502
McMillan DGG, Keis S, Berney M, Cook GM. Non-Fermentative Thermoalkaliphilic Growth is Restricted to Alkaline Environments. Appl Environ Microbiol 2009 Dec;75:7649-7654
Cook GM, Berney M, Gebhard S, Heinemann M, Cox RA, Danilchanka O, Niederweis M. Physiology of Mycobacteria. Advances in Microbial Physiology 2009;55:81-182, 318-9
Bosshard F., Berney M., Scheifele M., Weilenmann HU, Egli T. Solar disinfection (SODIS) and subsequent dark storage of S. typhimurium and S. flexneri monitored by flow cytometry. Microbiology-SGM 2009 Apr;155:1310-1317
Berney M, Vital M, Hülshoff I, Weilenmann HU, Egli T, Hammes F. Rapid, cultivation-independent assessment of microbial viability in drinking water. Water Res. 2008 Aug;42(14):4010-8. Epub 2008 Jul 22.
Hammes F, Berney M, Wang Y, Vital M, Köster O, Egli T. Flow-cytometric total bacterial cell counts as a descriptive microbiological parameter for drinking water treatment processes. Water Res. 2008 Jan;42(1-2):269-77. Epub 2007 Jul 14.
Berney M, Hammes F, Bosshard F, Weilenmann HU, Egli T. Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry. Appl Environ Microbiol. 2007 May;73(10):3283-90. Epub 2007 Mar 23.
Berney M, Weilenmann HU, Egli T. Adaptation to UVA radiation of E. coli growing in continuous culture. J Photochem Photobiol B. 2007 Feb 1;86(2):149-59. Epub 2006 Oct 19.
Berney M, Weilenmann HU, Simonetti A, Egli T. Efficacy of solar disinfection of Escherichia coli, Shigella flexneri, Salmonella Typhimurium and Vibrio cholerae. J Appl Microbiol. 2006 Oct;101(4):828-36.
Berney M, Weilenmann HU, Egli T. Gene expression of Escherichia coli in continuous culture during adaptation to artificial sunlight. Environ Microbiol. 2006 Sep;8(9):1635-47.
Berney M, Weilenmann HU, Egli T. Flow-cytometric study of vital cellular functions in Escherichia coli during solar disinfection (SODIS). Microbiology-SGM. 2006 Jun;152(Pt 6):1719-29.
Berney M, Weilenmann HU, Ihssen J, Bassin C, Egli T. Specific growth rate determines the sensitivity of Escherichia coli to thermal, UVA, and solar disinfection. Appl Environ Microbiol. 2006 Apr;72(4):2586-93.
Looser V, Hammes F, Keller M, Berney M, Kovar K, Egli T. Flow-cytometric detection of changes in the physiological state of E. coli expressing a heterologous membrane protein during carbon-limited fedbatch cultivation. Biotechnol Bioeng. 2005 Oct 5;92(1):69-78.
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Albert Einstein College of Medicine
Michael F. Price Center
1301 Morris Park Avenue , Room 522
Bronx, NY 10461