Associate Professor, Department of Biochemistry
The research in the Wu Lab integrates synthetic chemistry with biochemistry to explore the relevance of glycosylation in human disease. The glycome, defined as the full complement of glycans that a cell produces, is involved in a variety of physiological processes, including angiogenesis, fertilization, stem cell development and neuronal development. Changes in the glycome have also been shown to mark the onset of cancer and inflammation. The wealth of biological information encoded in the glycome has motivated researchers to develop methods for its retrieval.
Produced by the secondary metabolism rather than encoded in the genome, glycans are assembled in a step-wise fashion by multiple enzymes and thus by multiple genes. Therefore, genetic and biochemical tools alone cannot be used to define all aspects of the glycome. Rather, many complementary approaches must be applied in parallel in order to assemble a picture of the glycome both from the “bottom up” and from the “top down”.
The major goal of our lab is to develop chemical biology platforms to image and characterize the glycome in cancer cells and living organisms. We are also interested in chemical tools that can be used to selectively enrich glycoproteins for their molecular identification. These new tools will facilitate the discovery of new biomarkers for diseases, and assist in the development of clinical diagnostics and therapeutics.
Negative Feedback Regulation of Wnt Signaling via N-linked Fucosylation in Zebrafish. Feng, L.; Jiang, H.; Wu, P., Marlow, F.L. Dev. Biol. 2014, 395, 268.
Discovery of Autophagy Inhibitors with Anti-proliferative Activity in Lung and Pancreatic Cancer Cells. Nordstrøm, L.; Sironi, J.; Aranda, E.; Maisonet, J.; Perez-Soler, R.; Wu, P.; Schwartz, E. ACS. Med. Chem. Lett. 2015.
Detection and Isolation of Dendritic Cells Using Lewis X-functionalized Magnetic Nanoparticles. Rouhanifard, S. H.; Xie, R.; Zhang, G.; Sun, X.; Chen, X.; Wu, P. Biomacromolecules. Accepted.
Metabolic Labeling of Fucosylated Glycoproteins in Bacteroides. Webler-Besanceney, C.; Jiang, H.; Wang, W.; Baughn, A.; Wu, P. Bioorg. Med. Chem. Lett. The special issue in honor of Prof. Carolyn Bertozzi on the occasion of her receiving Tetrahedron Young Investigator Award. 2011, 21, 4989.
Tracking N-acetyllactosamine on Cell Surface Glycans in Vivo. Zheng, T.; Jiang, H.; Gros, M., Soriano del Amo, D.; Sundaram, S.; Lauvau, G.; Marlow, F.; Liu, Y., Stanley, P.; Wu, P. Angew. Chem. Int. Ed.2011, 50, 4113.
Biocompatible Copper(I) Catalysts for in Vivo Imaging of Glycans. Soriano del Amo, D.; Wang, W.; Hao, J.; Besanseney, C.; Yan, A, C.; Levy, M.; Liu, Y.; Marlow, F. Wu, P. J. Am. Chem. Soc. 2010, 132, 16893. Highlighted in C&En News 2010, 88 (48), 37.
Chemoenzymatic Synthesis of GDP-L-fucose and the Lewis X Glycan Derivatives. Wang, W.; Hu, T.; Frantom, P. A.; Zheng, T; Gerwe, B.; Soriano del Amo, D.; Seidel, R.D. III; Wu, P. Proc. Natl. Acad. Sci. USA. 2009,106, 16096.
Probe the Sialic Acid Biosynthetic Pathway Using Alkyne-Bearing Sugars. Chang, P.; Chen, X.; Smyrniotis, C.; Hu, T.; Bertozzi, C. R.; Wu, P. Angew. Chem. Int. Ed. 2009, 48, 4030.
Targeted Metabolic Labeling of Yeast N-glycans with Unnatural Sugars. Breidenbach, M. A.; Gallagher, J. E. G.; King, D. S.; Smart, B. P.; Wu, P.; Bertozzi, C. R. Proc. Natl. Acad. Sci. USA. 2010, 107, 3988.
The glycopeptide preferring polypeptide- GalNAc transferase-10 (ppGalNAc T10), involved in mucin type-O-glycosylation, has a unique GalNAc-O-Ser/Thr binding site in its catalytic domain not found in ppGalNAc T1 or T2. Perrine, C. L.; Ganguli, A; Wu, P.; Bertozzi, C. R. ; Fritz, T.A.; Raman, J.; Tabak, L. A.; Gerken, T. A. J. Biol. Chem. 2009, 284,20387.
Site-specific Chemical Modification of Recombinant Proteins Produced in Mammalian Cells Using the Genetically Encoded Aldehyde Tag. Wu, P.; Shui, W.; Carlson, B.; Hu, N.; Rabuka, D.; Lee, J.; Bertozzi, C. R. Proc. Natl. Acad. Sci. USA. 2009, 106, 3000.
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More Information About Dr. Peng Wu
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Albert Einstein College of Medicine
Michael F. Price Center
1301 Morris Park Avenue , Room 522
Bronx, NY 10461
Chemical & Engineering News highlights research by Drs. Peng Wu and Ben Ovryn, who devised a way to track the movement of single glycoprotein molecules on the surfaces of living cancer cells.