Faculty Profile

Dr. David R. Entenberg, M.Sc.

David R. Entenberg, M.Sc.

Senior Associate, Department of Anatomy & Structural Biology

Director of Technological Development, Intravital Imaging Program and Gruss-Lipper Biophotonics Center, Department of Anatomy & Structural Biology

Professional Interests

My expertise lies in the design and development of novel instrumentation and imaging technologies for biological research.  With a background in laser based experimental quantum physics I brings skills in optical, mechanical, electrical, software and instrument design.

My previous work has included the design and development of several robotics-based high-throughput automated biological assays utilizing real time PCR and MALDI-TOF protocols, novel microscopes including a fast switching, muti-channel TIRF microscope, a video rate multiphoton microscope, and a two-laser, OPO based multiphoton microscope and several image analysis software packages to facilitate the analysis of cell motility and colocalization image data. 

My current projects have focused on developing and optimizing surgical techniques for expanding the utility of mouse models within intravital imaging.  This work has included mammary, abdominal, brain, lymphatic and lung imaging windows for studying cancer metastasis to secondary sites in vivo, as well surgically implantable photolithographic microdevices designed to directly alter the local (<300um) tumor microenvironment in vivo and during intravital imaging.

Selected Publications

  1. Arwert E, Harney AS, Entenberg D, Wang AY, Sahai E, Pollard JW, Condeelis J. (2016). Spatial and temporal aspects of macrophage subsets required for tumour cell intravasation. Cancer Research. Submitted
  2. Harney AS, Pignatelli J, Smith BD, Kadioglu E, Wise SC, Hood MM, Kaufman MD, Leary CB, Liu W-P, Al-Ani GC, X, Entenberg D, Oktay MH, Wang Y, Chun L, De Palma M, Jones JG, Flynn DL, Condeelis J. (2016). Rebastinib inhibits recruitment and function of TIE2 macrophages in metastatic breast cancer. Science Translational Medicine. In Revision.
  3. Karagiannis GS, Pastoriza JM, Wang AY, Harney AS, Entenberg D, Pignatelli J, Jones JG, Anampa J, Sparano JA, Rohan TE, Condeelis J, Oktay MH. (2016 ). Taxane chemotherapy induces Mena- and TMEM-mediated pro-metastatic changes in breast cancer microenvironment. Nature Medicine. In Revision.
  4. Williams J, Entenberg D, Wang Y, Padgen M, Clark A, Castracane J, Condeelis J. (2016). Validation of a device for the active manipulation of the tumor microenvironment during intravital imaging. Intravital. 5(2):e1182271.  PMID: 27790386 / PMCID: PMC5079288. (Co-First Author Publication)
  5. Wang H, Wang Y, Li J, Entenberg D, Wyckoff J, Yuan Z, Wang W, Condeelis J. (2016). Direct visualization of single hypoxic tumor cells in vivo using a new hypoxia probe. Intravital. 5(2):e1187803.  PMID: 27790387 / PMCID: PMC5079291.
  6. Szulczewski JM, Inman DR, Entenberg D, Aguirre-Ghiso JA, Castracane J, Condeelis J, Elicieri KW, Keely P. (2016). In Vivo Visualization of Stromal Macrophages via label-free FLIM-based metabolite imaging. Scientific Reports. 6:25086.  PMID: 27220760 / PMCID: PMC4879594.
  7. Rodriguez-Tirado C, Kitamura T, Kato Y, Pollard JW, Condeelis J, Entenberg D. (2016). Long-term high-resolution intravital microscopy in the lung with a vacuum stabilized imaging window. Journal of Visualized Experiments. (116). PMID: 27768066.
  8. Harper K, Sosa MS, Hosseini H, Entenberg D, Avivar-Valderas A, Nagi C, Davis RJ, Farias EF, Condeelis J, Klein C, Aguirre-Ghiso JA. (2016). Mechanism of early dissemination and metastasis by ErbB2+ breast “pre-malignant” cells. Nature. In Press.
  9. Harney AS, Condeelis J, Entenberg D. (2015). Extended time-lapse intravital imaging of real-time multicellular dynamics in the tumor microenvironment. Journal of Visualized Experiments. (112):e54042.  PMID: 27341448 / PMCID: PMC4927790.
  10. Fluegen G, Avivar-Valderas A, Wang YR, Padgen MR, Williams JK, Nobre AR, Calvo V, Cheung JF, Bravo-Cordero JJ, Entenberg D, Castracane J, Verkhusha V, Keely PJ, Condeelis J, Aguirre-Ghiso JA. Disseminated tumor cell phenotypic heterogeneity is preset by primary tumo hypoxic microenvironments. Nature Cell Biology. In Press
  11. Zolla V, Nizamutdinova IT, Scharf B, Clement CC, Maejima D, Akl T, Nagai T, Luciani P, Leroux JC, Halin C, Stukes S, Tiwari S, Casadevall A, Jacobs WR, Jr., Entenberg D, Zawieja DC, Condeelis J, Fooksman DR, Gashev AA, Santambrogio L. (2015). Aging-related anatomical and biochemical changes in lymphatic collectors impair lymph transport, fluid homeostasis, and pathogen clearance. Aging Cell. 14(4):582-94.  PMID: 25982749 / PMCID: PMC4531072.
  12. Patsialou A, Wang Y, Pignatelli J, Chen X, Entenberg D, Oktay M, Condeelis JS. (2014). Autocrine CSF1R signaling mediates switching between invasion and proliferation downstream of TGFbeta in claudin-low breast tumor cells. Oncogene.  PMID: 25088194 / PMCID: PMC25088194
  13. Lim SJ, Zahid MU, Le P, Ma L, Entenberg D, Harney AS, Condeelis J, Smith AM. (2015). Brightness-equalized quantum dots. Nature Communications. 6:8210. PMID: 26437175 / PMCID: PMC4594210.
  14. Harney AS, Arwert EN, Entenberg D, Wang Y, Guo P, Qian BZ, Oktay MH, Pollard JW, Jones JG, Condeelis JS. (2015). Real-Time Imaging Reveals Local, Transient Vascular Permeability, and Tumor Cell Intravasation Stimulated by TIE2hi Macrophage-Derived VEGFA. Cancer Discovery. 5(9):932-43.  PMID: 26269515 / PMCID: PMC4560669.
  15. Entenberg D, Rodriguez-Tirado C, Kato Y, Kitamura T, Pollard JW, Condeelis J. (2015). Subcellular resolution optical imaging in the lung reveals early metastatic proliferation and motility. Intravital. 4(3).  PMID: 26855844 / PMCID: PMC4737962.
  16. Sharma VP, Entenberg D, Condeelis J. (2013). High-resolution live-cell imaging and time-lapse microscopy of invadopodium dynamics and tracking analysis. Methods in Molecular Biology. 1046:343-57. PMID: 23868599 / PMCID: PMC3933219.
  17. Sharma VP, Eddy R, Entenberg D, Kai M, Gertler FB, Condeelis J. (2013). Tks5 and SHIP2 regulate invadopodium maturation, but not initiation, in breast carcinoma cells. Current Biology. 23(21):2079-89.  PMID: 24206842 / PMCID: PMC3882144.
  18. Patsialou A, Bravo-Cordero JJ, Wang Y, Entenberg D, Liu H, Clarke M, Condeelis JS. (2013). Intravital multiphoton imaging reveals multicellular streaming as a crucial component of in vivo cell migration in human breast tumors. Intravital. 2(2):e25294.  PMID: 25013744 / PMCID: PMC3908591.
  19. Entenberg D, Kedrin D, Wyckoff J, Sahai E, Condeelis J, Segall JE. (2013). Imaging tumor cell movement in vivo. Current Protocols in Cell Biology. Chapter 19:Unit19 7.  PMID: 23456602 / PMCID: PMC3722061.
  20. Das S, Sarrou E, Podgrabinska S, Cassella M, Mungamuri SK, Feirt N, Gordon R, Nagi CS, Wang Y, Entenberg D, Condeelis J, Skobe M. (2013). Tumor cell entry into the lymph node is controlled by CCL1 chemokine expressed by lymph node lymphatic sinuses.     . 210(8):1509-28.  PMID: 23878309 / PMCID: PMC3727324.
  21. Subach OM, Entenberg D, Condeelis JS, Verkhusha VV. (2012). A FRET-facilitated photoswitching using an orange fluorescent protein with the fast photoconversion kinetics. Journal of the American Chemical Society. 134(36):14789-99.  PMID: 22900938 / PMCID: PMC3444247.
  22. Hulit J, Kedrin D, Gligorijevic B, Entenberg D, Wyckoff J, Condeelis J, Segall JE. (2012). The use of fluorescent proteins for intravital imaging of cancer cell invasion. Methods in Molecular Biology. 872:15-30. PMID: 22700401 / PMCID: PMC4000026.
  23. Wyckoff J, Gligorijevic B, Entenberg D, Segall J, Condeelis J. (2011). High-resolution multiphoton imaging of tumors in vivo. Cold Spring Harb Protoc. 2011(10):1167-84.  PMID: 21969629.
  24. Wyckoff J, Gligorijevic B, Entenberg D, Segall J, Condeelis J. (2011). The in vivo invasion assay: preparation and handling of collection needles. Cold Spring Harb Protoc. 2011(10):1232-4.  PMID: 21969630.
  25. Entenberg D, Wyckoff J, Gligorijevic B, Roussos ET, Verkhusha VV, Pollard JW, Condeelis J. (2011). Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging. Nature protocols. 6(10):1500-20.  PMID: 21959234.
  26. Dovas A, Gligorijevic B, Chen X, Entenberg D, Condeelis J, Cox D. (2011). Visualization of actin polymerization in invasive structures of macrophages and carcinoma cells using photoconvertible beta-actin-Dendra2 fusion proteins. PloS one. 6(2):e16485.  PMID: 21339827 / PMCID: PMC3038862.
  27. Wyckoff J, Gligorijevic B, Entenberg D, Segall JE, Condeelis J. (2010). High-Resolution multiphoton imaging of Tumors in vivo. Live Cell Imaging: A Laboratory Manual.441-61.  / PMCID: PMC4000043.
  28. Gligorijevic B, Entenberg D, Kedrin D, Segall J, van Rheenen J, Condeelis J. (2010). Intravital Imaging and Photoswitching in Tumor Invasion and Intravasation Microenvironments. Micros Today. 18(1):34-7.  PMID: 25635177 / PMCID: PMC4307802.
  29. Gligorijevic B, Entenberg D, Kedrin D, Segall J, van Rheenen J, Condeelis J. (2009). Intravital imaging and photoswitching in tumor invasion and intravasation microenvironments. Microscopy and Microanalysis. Supplement 2(15):86-7.
  30. Dunphy MP, Entenberg D, Toledo-Crow R, Larson SM. (2009). In vivo microcartography and subcellular imaging of tumor angiogenesis: a novel platform for translational angiogenesis research. Microvascular research. 78(1):51-6.  PMID: 19362098 / PMCID: PMC2739383.
  31. Entenberg D, Aranda I, Li Y, Toledo-Crow R, Schaer D, Li Y. (2006). Multimodal microscopy of immune cells and melanoma for longitudinal studies. Proc of SPIE. 6081:62-73.
  32. Villanueva J, Philip J, Entenberg D, Chaparro CA, Tanwar MK, Holland EC, Tempst P. (2004). Serum peptide profiling by magnetic particle-assisted, automated sample processing and MALDI-TOF mass spectrometry. Analytical chemistry. 76(6):1560-70.  PMID: 15018552.
  33. Entenberg D, Roorda RD, Toledo-Crow R. Non-linear microscope for imaging of the neural systems in live drosophila.  OSA BioMed 2004.


 In Press.

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