Science at the heart of medicine

7023 Phenotyping of Genetically Engineered Mice and Mouse Models of Disease

COURSE DESCRIPTION: This course is intended to familiarize graduate students in the issues associated with the development and interpretation of genetically engineered mice. Specifically, the course will deal with:

Mouse and gene nomenclature with introduction to web resources

- Mouse biology and physiology with emphasis on background strain, infectious disease, and environmental influences on the mouse phenotype
- Techniques for the development of genetically engineered mice
- Clinical and Anatomic Pathology phenotyping with focus on criteria for sacrifice and necropsy, necropsy and fixation considerations, special techniques (immunohistochemistry,
  in situ, FISH, special stains, etc.), and common background findings.
- Behavioral and motor phenotyping of genetically engineered mice
- Metabolic and Endocrine Phenotyping
- Cardiovascular phenotyping
- Approaches for understanding and managing early embryonic death
- In vivo phenotyping with the use of various imaging modalities such as PET, intravital microscopy, echocardiography, and EGFP imaging modalities
- Post-mortem imaging modalities, EM, Confocal
- The use of alternative species as translational research models (zebrafish)



STUDENT PREPARATION: Ideally the students would be graduate students or post-docs who are presently or will be utilizing in vivo models.

SUITABLE FOR 1ST YEAR STUDENTS: Yes. Although the course is open to all Einstein graduate and MSTP students, it is particularly recommended to those who have completed their first year and have initiated research within the lab of their mentor.

UNIQUE TRAINING OFFERED IN THIS COURSE: This course is to familiarize graduate students with the evaluation of genetically engineered mice and mouse models of disease. The lectures topics include mouse nomenclature and strain variation, diseases, imaging, generating GEMS, and phenotyping approaches (pathology, cardiovascular, behavioral, embryo, etc.)

STUDENT ASSESSMENTS: Mid-term and final