Marion Bessin Liver Research Center

Imaging and Cell Structure Core

6th Floor Forchheimer

Erik Lee Snapp, Ph.D.
Director
Associate Professor
Department of Anatomy and Structural Biology


Frank Macaluso, M.Sc.
Training Director and Senior Associate


Purpose 

The Imaging and Cell Structure Core operates in conjunction with the Analytical Imaging Facility to provide a state-of-the-art resource for modern imaging and computer analysis techniques.

The Core facilitates use of imaging techniques in liver research by providing specialized instrumentation, such as laser scanning confocal microscopy, two photon microscopy, immunocryoelectron microscopy, and expertise in specialized procedures utilizing such instrumentation such as total internal reflection microscopy (TIRF), fluorescence resonance energy transfer (FRET), and fluorescence recovery after photobleaching (FRAP). Technical support for assisted or independent use of such instrumentation is provided. Assistance in planning experiments to utilize imaging procedures, and in light and electron microscopic data interpretation, is available. An extensive selection of fluorescent protein vectors, labeling technologies, and consulting in the design of fluorescent fusion protein chimeras is available. Please contact Erik Lee Snapp (erik-lee.snapp@einstein.yu.edu) to schedule a consultation.


Services 

  1. Image Analysis: High Resolution Microscopy
    • Perkin Elmer Spinning Disk Confocal Microscope with photomanipulation attachment
    • Leica AOBS SP2 and SP5 Laser Scanning Confocal Microscopes
    • Zeiss Live/DUO Confocal Microscope
    • Multi-Photon Invervital Imaging Microscopy
    • Correlative Microscopy with Zeiss Shuttle system
     
  2. Image Analysis: Other Light Microscopic Techniques
    • Volume Rendering and 3D Quantitation
    • D.I.C. (Nomarski), Darkfield, Phase Contrast, IRM, and Epifluorescence with digital imaging
    • Deconvolution (Deltavision microscope)
    • Particle and Cell Tracking
    • Microinjection
    • Fluorescence Resonance Energy Transfer
    • FRAP, FLIP, Photoactivation, iFRAP, and other photomanipulation techniques
    • Total Internal Reflection Fluorescence Microscopy (TIRF)
     
  3. Analytical Procedures: Image Digitalization and Quantification
    • Computer Support and Networking, Glossy prints
    • Time Lapse Motion Analysis
    • Colocalization
    • Diffusion analysis
     
  4. Electron Microscopy
    • JEOLCO 100CXII Transmission Electron Microscope
    • JEOL 1200EX
    • Cryoultramicrotome
    • Cryo Electron Microscopy
    • JEOLCO 6400 Scanning Electron Microscope
    • Cressington Freeze Etch Unit
     
  5. Histological Services
    • Consultation and training on technical approaches, dissection, and tissue preparation
    • Histopathology interpretation
    • Optimization of histochemistry and immunohistochemistry
    • General immunohistochemistry
    • Histopathological analysis of embryonic tissues
    • Electron microscopy interpretation
    • Human liver tissue bank
     
  6. Cytological and Histochemical and Cytochemical Procedures
    • Including, but not limited to, enzyme cytochemistry, immunocytochemistry, in situ hybridization and autoradiography at both light and electron microscopic levels
     
  7. Fluorescent Protein Resource Center: fluorescent protein plasmids library, expression vectors, fluorescent dye-labeled secondary antibodies, fluorescent organelle markers 

Contact 

Erik Lee Snapp, Ph.D.
Associate Professor
Director, Imaging and Cell Structure Core of the Marion Bessin Liver Center
Department of Anatomy and Structural Biology
Albert Einstein College of Medicine
1300 Morris Park Avenue
Bronx, NY 10464

718-430-2967
Fax 718-430-8996

Snapp Laboratory 

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