Marion Bessin Liver Research Center

Imaging and Cell Structure Core

6th Floor Forchheimer

Carolina Eliscovich, Ph.D.
Director


Frank Macaluso, M.Sc.
Training Director and Senior Associate


Purpose 

The Imaging and Cell Structure Core of the Liver Research Center operates in conjunction with the Analytical Imaging Facility (AIF) to provide a state-of-the-art resource for modern fluorescence and electron microscopy for Einstein Investigators.

The Core facilitates use of imaging techniques in liver research by supporting and assisting with use of specialized instrumentation, including laser scanning confocal microscopy, deconvolution microscopy, multi-photon microscopy, cryo-electron microscopy (cryo-EM) and correlative light electron microscopy (CLEM). In addition, the Core provides expertise in and assistance with specialized imaging techniques such as correlative microscopy, vesicle tracking, volumetric measurements, ultrastructural sample preparation, super resolution fluorescence microscopy (STORM/SIM), Fluorescence Recovery After Photobleaching (FRAP), Fluorescence Resonance Energy Transfer (FRET) and Total Internal Reflection Microscopy (TIRF).

The Core provides technical support for assisted or independent use of the imaging technology and helps in the design of experiments and data analysis related with relevant studies on hepatocytes and liver tissue.

An extensive selection of fluorescent protein vectors, fluorescent antibodies and probes, cell dye labeling technologies, and advise in the design of light and electron microscopy experiments relevant to hepatocytes and liver tissue is available.


Services 

  1. High Resolution Microscopy
    • Perkin Elmer Spinning Disk Confocal Microscope with photo manipulation attachment
    • Leica AOBS SP2 and SP5 Laser Scanning Confocal Microscopes
    • Zeiss Live/DUO Confocal Microscope
    • Olympus Fluoview Multi-Photon Intravital Imaging Microscopy
    • Correlative Microscopy with Zeiss Shuttle and Find System
    • Nikon Super Resolution Microscope (TIRF, 2D-STORM, 3D-STORM, 3D-SIM)
    • PerkinElmer P250 High Capacity Slide Scanner (Brightfield and Fluorescence)
     
  2. Other Light Microscopic Techniques
    • Volume Rendering and 3D Quantitation
    • D.I.C. (Nomarski), Darkfield, Phase Contrast, IRM, and Epifluorescence with digital imaging
    • Deconvolution (DeltaVision microscope)
    • Particle and Cell Tracking
    • Microinjection
    • Förster Resonance Energy Transfer (FRET)
    • FRAP, FLIP, Photoactivation, iFRAP, and other photo manipulation techniques
    • Total Internal Reflection Fluorescence Microscopy (TIRF)
     
  3. Analytical Procedures: Image Digitalization and Quantification
    • Computer Support and Networking
    • Time Lapse Motion Analysis
    • Colocalization
    • Diffusion analysis
     
  4. Electron Microscopy
    • JEOL 100CXII and JEOL 1200EX Transmission Electron Microscopes
    • FEI Tecnai 20 Cryo Electron Microscope
    • Zeiss Supra 40 Field Emission Scanning Electron Microscope
    • Cryo-ultramicrotome
    • Cressington Freeze Etch Unit
     
  5. Histological Services (in conjunction with the Histology & Comparative Pathology Facility)
    • Consultation and training on technical approaches, dissection, and tissue preparation
    • Histopathology interpretation
    • Optimization of histochemistry and immunohistochemistry
    • General immunohistochemistry
    • Histopathological analysis of embryonic tissues
    • Electron microscopy interpretation
    • Human liver tissue bank
     
  6. Cytological and Histochemical and Cytochemical Procedures
    • Including, but not limited to, enzyme cytochemistry, immunocytochemistry, in situ hybridization and autoradiography at both light and electron microscopic levels.
     
  7. Fluorescent Protein Resource Center: fluorescent protein plasmids library, expression vectors, fluorescent dye-labeled secondary antibodies, fluorescent organelle markers

Contact 

For additional information, please contact Carolina Eliscovich, Ph.D

Golding 601

Phone 718.430.8597

E-mail: carolina.eliscovich@einstein.yu.edu 

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