Spotlight

Rose F. Kennedy Intellectual and Developmental Disabilities Research Center

Tissue Engineering and Cellular Reprogramming (TECR) Core

Director, TECR Core: Dr. Mark Mehler
Associate Directors, TECR Core: Dr. Solen Gokhan and Dr. Aldrin E. Molero

The Tissue Engineering and Cellular Reprogramming (TECR) Core provides innovative and state-of-the-art interdisciplinary expertise, equipment, essential molecular, cellular and systems-level reagents and associated equipment and dedicated space techniques for the study of cell and tissue preparations from both human and animal models of intellectual and developmental disorders. These services are designed to facilitate research projects employing a wide variety of experimental approaches ranging from the use of cellular and molecular biology techniques, genomic, epigenomic and systems biological analyses and neurophysiological approaches to the study of distinct animal and human cellular and tissue preparations. The core supplies these services in conjunction with other RFK IDDRC core facilities and specialized support services by providing extensive training, oversight, facilities and novel and unique resources to expedite these interdisciplinary goals.

Objectives

  1. To provide state-of-the-art equipment and expertise to facilitate the study of a broad variety of cell and tissue types from individuals with intellectual and developmental disabilites as well as from cell and animal models
  2. To facilitate combined use of in vitro and in vivo approaches to study individual genes and epigenetic modulators during brain development in health and genetic disease
  3. To develop novel therapeutic strategies and promote neural cell protection and/or neural regeneration through genetic and epigenetic cellular reprogramming and tissue engineering

Resources/Services

  • In vitro dissociated cell culture, slice and organotypic and whole embryo cultures
  • Embryonic stem (ES) cell experimental applications
  • Viral vector cellular manipulations for in vitro and in vivo applications
  • Human fetal cell experimentation
  • Fluorescence-activated cell sorting (FACS) for analytical and preparatory experimental approaches
  • Quantitative analysis of gene expression

Fees

Facility Cost for IDDRC Member Cost for Non-IDDRC Member
In Vitro Tissue Culture & ESC Facilities:
-Training to perform clonal murine & human dissociated, slice, organotypic, iPS and direct somatic reprogrammed cell techniques
-Generation of specific developmental cell types
-Procurement of murine ES cell lines
-Generation of murine & human iPS and direct somatic cell lines
 
 
$200/4-hour session
$500/paradigm
$150/cell line
$1,000-3,200/cell line (depending on the number of nonroutine viral constructs and feeder cells required)
 
$300/4-hour session
$750/paradigm
$225/cell line
$1,500-4,800/cell line (depending on the number of nonroutine viral constructs and feeder cells required)
In Vivo Embryonic, Postnatal and Adult Surgical Facilities:
-Training to perform ultrasound–guided implantation into embryonic mouse brain
-Training in use of stereotactic surgical apparatus (postnatal and adult)
-Unassisted use of embryonic brain surgical suite
-Unassisted use of stereotactic surgical apparatus
 
 
$350/3-hour session
$200/half-day session
$250/3-hour session
$100/half-day session
 
$525/3-hour session
$300/half-day session
$375/3-hour session
$150/half-day session
Molecular Reagent Construction and Viral Vector Facility:
Engineering and synthesis of targeting vector constructs
 
 
$3,000/vector
 
$4,000/vector
FACS Facility:
Assisted use only
 
$65/hr
 
$85/hr

 

Tissue Engineering and Cellular Reprogramming (TECR) Core:

To provide a wide variety of cellular and molecular biology techniques for the study of cell and tissue preparations as applied to both human and animal models of IDD; Shared Facilities 

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