The overall goal of the Einstein-Montefiore CFAR Biohazard Animal Core is to enable Einstein investigators, through the provision of a safe and effective facility and a highly trained support staff, to apply the animal modeling approach to pathogen research with HIV and AIDS-related opportunistic pathogens that requires bio-containment.
- To provide a biohazard containment laboratory that is outfitted with state-of-the-art apparatus and equipment for safe, efficient, and in-depth analysis of Biosafety-Level-2/3 (BSL2/3) pathogens using appropriate animal models.
- To provide assistance for researchers in the design of experiments involving in vivo modeling of BSL2/3 pathogens, in the choice of suitable mouse strains (including supplying specialized transgenic and knockout mice), in carrying out experimental procedures, in sample collection and processing, as well as in data interpretation.
- To enable CFAR investigators, particularly those inexperienced in animal studies, to extend their research programs to include in vivo
modeling of infection with HIV and AIDS-associated pathogens.
- BSL3-level containment for housing infected mice
- BSL3-containment aerosolization infection units
- Animal Behavioral Study Facility
- In Vivo Bioluminescent and Fluorescent Imaging Facility
- Technical support for animal modeling of infection with HIV and HIV-related pathogens.
- Infection of mice by aerogenic challenge with Mtb including MDR and XDR Mtb isolates.
- Infection of mice by intra-cranial inoculation
- Transgenic and humanized mouse models to study HIV infection
Core Training Programs
- Performance of animal procedures under appropriate biohazard containment.
- Administration of pharmacological or biological agents via various routes.
- Necropsy and dissection of mice to harvest infected tissues.
- Tissue preparation methods for immunological, pathological and molecular analysis
- Infection of mice by aerogenic challenge: Technician time and supplies, $50 per hour;
- Infection of mice by intra-cranial inoculation: Technician time and supplies: $30 per hour;
- Humanized mice: Thy/Liv-SCID-hu mice, $200 each; HSC-NSG mice, $100 each
- Specialty Animal strains: NOD/SCID/IL2R (NSG) knockout mice: $40 each, Rag2-/- gc-/- mice: $60 each.
To access Core services please contact Dr. John Chan
Recent Core Publications
1. Nixon B, Fakioglu E, Stefanidou M, Wang Y, Dutta M, Goldstein H, Herold BC. Genital Herpes Simplex Virus Type 2 Infection in Humanized HIV-Transgenic Mice Triggers HIV Shedding and Is Associated With Greater Neurological Disease. J Infect Dis. 2013. Epub 2013/08/31. doi: 10.1093/infdis/jit472. PubMed PMID: 23990571.
2. Nixon B, Stefanidou M, Mesquita PM, Fakioglu E, Segarra T, Rohan L, Halford W, Palmer KE, Herold BC. Griffithsin protects mice from genital herpes by preventing cell-to-cell spread. J Virol. 2013;87(11):6257-69. Epub 2013/03/29. doi: 10.1128/JVI.00012-13. PubMed PMID: 23536670; PubMed Central PMCID: PMC3648100.
3. Seay K, Qi X, Zheng JH, Zhang C, Chen K, Dutta M, Deneroff K, Ochsenbauer C, Kappes JC, Littman DR, Goldstein H. Mice Transgenic for CD4-Specific Human CD4, CCR5 and Cyclin T1 Expression: A New Model for Investigating HIV-1 Transmission and Treatment Efficacy. PLoS ONE. 2013;8(5):e63537. Epub 2013/05/22. doi: 10.1371/journal.pone.0063537. PubMed PMID: 23691059; PubMed Central PMCID: PMC3655194.
4. Szymczak WA, Davis MJ, Lundy SK, Dufaud C, Olszewski M, Pirofski LA. X-linked immunodeficient mice exhibit enhanced susceptibility to Cryptococcus neoformans Infection. mBio. 2013;4(4). Epub 2013/07/04. doi: 10.1128/mBio.00265-13. PubMed PMID: 23820392; PubMed Central PMCID: PMC3705448.