Stable Isotope and Metabolomics

Overview

The Stable Isotope and Metabolomics Core can provide targeted and untargeted metabolite profiling of plasma or tissue, and the rate of change of substrates (flux), using stable isotopes and mass spectrometry. The core has a variety of in vitro and in vivo metabolic methodologies that can determine substrate flux dynamics and metabolite profiles at the organelle, cellular, tissue and whole body levels. In collaboration with other Diabetes Research Center cores, the application of mass spectrometric and other methodologies allows the elucidation of metabolic mechanisms underlying disorders in fuel homeostasis. The metabolic role of candidate molecules can be specifically delineated in vivo and in vitro, using a step-by-step hypothesis-driven approach in animals, cell lines and other models.

Services

  • Stable isotope substrate flux dynamics
  • Metabolite quantification and identification
  • Cellular rates of glycolysis and mitochondrial respiration using the Seahorse Biosciences Extracellular Flux Analyzer

Location and Contacts

Stable Isotope and Metabolomics

Price Center/Block Research Pavillion 368

 
 
 

Director
Irwin Kurland, M.D., Ph.D.
718.678.1091
irwin.kurland@einstein.yu.edu







Center Affiliations

Diabetes Research Center