Associate Professor, Department of Anatomy & Structural Biology
The research of our laboratory is focused on the development of advanced high-resolution and single-molecule microscopy techniques and their application to study how biomolecular motors work and generate biological motion. In particular, the Gennerich Lab combines single-molecule biophysics with cell biology and biochemistry to study the molecular mechanisms underlying cell division, intracellular organelle and mRNA transport, as well as the molecular mechanism of protein degradation. Current research is focused on the molecular function of the microtubule motor cytoplasmic dynein (a molecular machine that harnesses the chemical energy of ATP hydrolysis to perform mechanical work in eukaryotic cells) and its role in sister-chromatid separation and chromosome segregation, and the transport of mitochondria and mRNA. We use a multidisciplinary approach integrating ultrasensitive single-molecule assays (high-resolution optical trapping and single-molecule fluorescence microscopy), subwavelength resolution live cell imaging and genetic approaches such as homologous recombination and RNA interference to dissect the mechanisms of microtubule-based motor proteins and their associated biological processes. Our long-term goal is to understand the fundamental design principles of biomolecular motors and the molecular basis of human diseases with underlying defects in motor function.
Nicholas, M. P., F. Berger, L. Rao, S. Brenner, C. Cho, and A. Gennerich. Cytoplasmic dynein regulates its attachment to microtubules via nucleotide state-switched mechanosensing at multiple AAA domains. 2015. PNAS 12:6371-6376.
Nicholas, M. P.*, P. Höök*, S. Brenner, C. Lazar, R. B. Vallee#, and A. Gennerich#. Control of cytoplasmic dynein force production and processivity by its C-terminal domain. 2015. Nat. Commun. 6:6206 (*: Co-first authors; #: co-corresponding authors).
Nicholas, M. P., L. Rao, and A. Gennerich. An improved optical tweezers assay for measuring the force generation of single kinesin molecules. 2014. Methods Mol. Biol. 1136:171-246.
Nicholas, M. P., L. Rao, and A. Gennerich. Covalent immobilization of microtubules on glass surfaces for molecular motor force measurements and other single-molecule assays. 2014. Methods Mol. Biol. 1136:137-169.
Gennerich, A. Molecular Motors: DNA Takes Control. 2014. Nat. Nanotechnol. 9:11-12 (invited News & Views article).
Rao, L.*, Romes, E. M.*, M. P. Nicholas, S. Brenner, T. Ashutosh, A. Gennerich#, and K. C. Slep#. The yeast dynein dyn2-pac11 complex is a dynein dimerization/processivity factor: structural and single molecule characterization. 2013. Mol. Biol. Cell 24:2362-2377 (*: co-first authors; #: co-corresponding authors).
Shih, S.-M., F. Kocabas, B. Engel, A. Gennerich, W. Marshall, and A. Yildiz.Single-molecule analysis of intraflagellar transport in Chlamydomonas reinhardtii. 2013. eLife 2:e00744
Gennerich, A., and S. L. Reck-Peterson. Probing the force generation and stepping behavior of cytoplasmic dynein. 2011. Methods Mol. Biol. 783:63-80.
Reck-Peterson, S. L., R. D. Vale, and A. Gennerich. “Motile Properties of Cytoplasmic Dynein”. In: “Handbook of Dynein” (Pan Stanford Publishing). 2011. Editors: Linda Amos and Keiko Hirose.
Huckaba, T., A. Gennerich, J. E. Wilhelm, A. Chishti, and R. D. Vale. Kinesin 73 is a processive motor that localizes to Rab5-containing organelles. 2011. J. Biol. Chem. 286:7457-7467.
Yildiz, A., M. Tomishige, A. Gennerich, and R. D. Vale. Intramolecular strain coordinates kinesin stepping behavior along microtubules. 2008. Cell, 134:1030-1041.
Gennerich, A., A. P. Carter, S. L. Reck-Peterson, and R. D. Vale. Force-induced bidirectional stepping of cytoplasmic dynein. 2007. Cell 131:952-965.
Imanishi, M., N. F. Endres, A. Gennerich, and R. D. Vale. Autoinhibition regulates the motility of the C. elegans intraflagellar transport motor OSM-3. 2006. J. Cell Biol. 174:931-937.
Reck-Peterson, S. L., A. Yildiz, A. P. Carter, A. Gennerich, N. Zhang, and R. D. Vale. Stepping behavior and structural requirements for cytoplasmic dynein processivity. 2006. Cell 126:335-348.
Gennerich, A. and D. Schild. Finite-particle tracking reveals sub-microscopic size changes of mitochondria during transport in mitral cell dendrites. 2006. Phys. Biol. 3:45-53.
Gennerich, A. and D. Schild. Sizing-up finite fluorescent particles with nanometer-scale precision by convolution and correlation image analysis. 2005. Eur. Biophys. J. 34:181-99.
Gennerich, A. and D. Schild. Anisotropic diffusion in mitral cell dendrites revealed by fluorescence correlation spectroscopy. 2002. Biophys. J. 83:510-522.
Peters, F., A. Gennerich, D. Czesnik, and D. Schild. Low frequency voltage clamp: recording of voltage transients at constant average command voltage. 2000. J. Neurosci. Meth. 99:129-135.
Gennerich, A. and D. Schild. Fluorescence correlation spectroscopy in small cytosolic compartments depends critically on the diffusion model used. 2000. Biophys. J. 79:3294-3306.
More Information About Dr. Arne Gennerich
Material in this section is provided by individual faculty members who are solely responsible for its accuracy and content.
Albert Einstein College of Medicine
Jack and Pearl Resnick Campus
1300 Morris Park Avenue
Forchheimer Building, Room 628C
Bronx, NY 10461